An Open Access Resource for Marmoset Neuroscientific Apparatus.
Authors: Rollin IZ, Papoti D, Bishop M, Szczupak D, Corigliano MR, Hitchens TK, Zhang B, Pell SKA, Guretse SS, Dureux A, Murai T, Sukoff Rizzo SJ, Klassen LM, Zeman P, Gilbert KM, Menon RS, Lin MK, Everling S, Silva AC, Schaeffer DJ
The use of the common marmoset (<i>Callithrix jacchus</i>) for neuroscientific inquiry has grown precipitously over the past two decades. Despite windfalls of grant support from funding initiatives in North America, Europe, and Asia to model human brain diseases in the marmoset, marmoset-specific apparatus are of sparse availability from commercial vendors and thus are often developed and reside within individual laboratories. Through our collective research efforts, we have designed and vetted myriad designs for awake or anesthetized magnetic resonance imaging (MRI), positron emission tomography (PET), computed tomography (CT), as well as focused ultrasound (FUS), electrophysiology, optical imaging, surgery, and behavior in marmosets across the age-span. This resource makes these designs openly available, reducing the burden of de novo development across the marmoset field. The computer-aided-design (CAD) files are publicly available through the Marmoset Brain Connectome (MBC) resource (https://www.marmosetbrainconnectome.org/apparatus/) and include dozens of downloadable CAD assemblies, software and online calculators for marmoset neuroscience. In addition, we make available a variety of vetted touchscreen and task-based fMRI code and stimuli. Here, we highlight the online interface and the development and validation of a few yet unpublished resources: Software to automatically extract the head morphology of a marmoset from a CT and produce a 3D printable helmet for awake neuroimaging, and the design and validation of 8-channel and 14-channel receive arrays for imaging deep structures during anatomical and functional MRI.
Introduction
Purpose
Other
Study Objective
To create and openly share a centralized resource of validated marmoset-specific apparatus designs, software (including AMIHGOS), imaging coil designs, and behavioral tools to reduce duplicated development and improve reproducibility in marmoset neuroscience.
Animal model / Human subject
Common marmoset (Callithrix jacchus); strain: None; age: None; sex: None
Disease model
Healthy
Outcomes and Safety
Summary of Outcomes
Custom AMIHGOS-fitted helmets plus training rapidly reduced behavioral agitation (Behavioral Response Scale from ~4 to 1 by day 14) and minimized head motion during awake imaging (examples <100 μm translational), while newly developed 8-channel and 14-channel receive arrays produced high local SNR and supported parallel imaging (8‑channel: feasible GRAPPA acceleration factor 2 in left–right direction; 14‑channel: low mean noise correlation ~8.3% and robust whole‑volume sensitivity).
Duration of biological effect
20 minutes
Safety-related matter
No adverse effects were reported; the custom AMIHGOS helmets are non-invasive (avoiding surgically implanted chambers), significantly reduced translational and rotational head motion, and animals' behavioral stress scores improved from ~4 to 1 by day 14, indicating they were comfortable and secure.
Brain Region
Ultrasound Parameters
Focal Characteristics
Focal depth: None; Focal length: None; Aperture size: None
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