Non-invasive Low-Intensity Pulsed Ultrasound Modulates Primary Cilia of Rat Hippocampal Neurons.

Transcranial modulation of primary cilia may provide new opportunities in the treatment of neurodegenerative diseases. This study investigates the effect of non-invasive low-intensity pulsed ultrasound (LIPUS) stimulation on primary cilia of rat hippocampal neurons. Three hours of LIPUS stimulation significantly reduced the incidence rate and length of cilia on cultured neurons (p < 0.01). Similarly, increasing the duration and intensity of LIPUS stimulation decreased the incidence and length of cilia. LIPUS stimulation improved c-fos expression when it was delivered to CA1 of the intact hippocampus of rats. And prolonged LIPUS stimulation (frequency: 0.5 MHz, pulse repetition frequency: 500 Hz, duty cycle: 5%, I_spta: 255 mW/cm², 10 min/d, 10 d) caused a statistically significant reduction in the incidence rate (p < 0.05) and length of primary cilia (p < 0.01) of neurons in rat CA1 hippocampus. These results indicate the promising potential of LIPUS stimulation in the treatment of primary cilium-related brain diseases.