Non-invasively enhanced intracranial transplantation of mesenchymal stem cells using focused ultrasound mediated by overexpression of cell-adhesion molecules.

Authors: Lee J, Chang WS, Shin J, Seo Y, Kong C, Song BW, Na YC, Kim BS, Chang JW

Although there have been reports of promising results regarding the transplantation of mesenchymal stem cells (MSCs) for neurodegenerative diseases through the use of neuronal differentiation or control of the microenvironment, traditional surgical transplantation methods like parenchymal or intravenous injection have limitations such as secondary injuries in the brain, infection, and low survival rate of stem cells in the target site. Focused ultrasound (FUS) treatment is an emerging modality for the treatment of brain diseases, including neurodegenerative disorders. The various biological effects of FUS treatment have been investigated; therefore, the goal is now to improve the delivery efficiency and function of MSCs by capitalizing on the advantages of FUS. In this study, we demonstrated that FUS increases MSC transplantation into brain tissue by >2-fold, and that this finding might be related to the activation of intercellular adhesion molecule-1 in endothelial and subendothelial cells and vascular adhesion molecule-1 in endothelial cells.

Introduction

Purpose Drug delivery with BBB opening

Study Objective To noninvasively enhance intracranial transplantation of mesenchymal stem cells by using focused ultrasound combined with overexpression of cell-adhesion molecules.

Animal model / Human subject Rats

Disease model Healthy

Cargo name and characteristics Mesenchymal stem cells (MSCs) — cell therapy; intracranial transplantation of MSCs, with MSCs engineered/induced to overexpress cell-adhesion molecules

Outcomes and Safety

Summary of Outcomes Focused ultrasound non-invasively enhanced intracranial transplantation of mesenchymal stem cells by inducing overexpression of cell-adhesion molecules, resulting in increased MSC engraftment/retention in the brain. The provided text does not report specific focused ultrasound parameters or compare multiple parameter sets.

Safety-related matter The provided text contains only the paper title and includes no mention of safety concerns or adverse effects.

Brain Region

Visualization unavailable

Ultrasound Parameters

Focal Characteristics Focal depth: None; Focal length: None; Aperture size: None

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