Focused Ultrasound-Mediated Intranasal Brain Drug Delivery Technique (FUSIN).

Authors: Ye D, Chen H

The blood-brain barrier (BBB) is the major barrier for brain drug delivery and limits the treatment options for central nervous system diseases. To circumvent the BBB, we introduced the focused ultrasound-mediated intranasal brain drug delivery (FUSIN) technique. FUSIN utilizes the nasal route for direct nose-to-brain drug administration, bypassing the BBB and minimizing systemic exposure. It also uses the transcranial application of ultrasound energy focused at a targeted brain region to induce microbubble cavitation, which enhances the transport of intranasally administered agents at the FUS-targeted brain location. FUSIN is unique in that it can achieve noninvasive and localized brain drug delivery with minimized systemic toxicity. The goal of this chapter is to provide a detailed protocol for FUSIN delivery to the mouse brain.

Introduction

Purpose Drug delivery WITHOUT BBB opening

Study Objective To develop and evaluate a focused ultrasound-mediated intranasal technique (FUSIN) to enhance drug delivery to the brain.

Animal model / Human subject mouse, C57BL/6J, 8–10 weeks, male

Disease model healthy

Cargo name and characteristics nanoparticle

Route of administration intranasal

Outcomes and Safety

Summary of Outcomes FUSIN technique significantly enhanced the intranasal delivery of gold nanoparticles to the brain.

Duration of biological effect not reported

Safety-related matter No tissue damage, hemorrhage, or inflammation reported.

Brain Region

Visualization unavailable

Ultrasound Parameters

Ultrasound instrument single-element focused ultrasound transducer

FUS Frequency 1.5 MHz

FUS Intensity not reported

FUS Pressure 1.1 MPa

FUS Mode not reported

Pulse duration 10 ms

Duration of a single FUS session 120 s

Focal Characteristics 20 mm

We are open to feedback. If you see a mistake or have a suggestion, please contact us.