Cortical Hemodynamic Responses Under Focused Ultrasound Stimulation Using Real-Time Laser Speckle Contrast Imaging.
Authors: Yuan Y, Zhao Y, Jia H, Liu M, Hu S, Li Y, Li X
Although there is increasing use of focused ultrasound stimulation (FUS) in brain studies, the real-time changes of the cerebral blood flow (CBF) due to FUS remain unclear. In this study, we developed a novel scheme combining FUS and laser speckle contrast imaging, which can be used to measure the CBF caused by FUS in real time. The results showed that the change of CBF increased from 0 to 30 s and reached up to the maximum of 115.1 ± 6.5% at 30 s and then decreased gradually from 30 to 60 s. This study demonstrates that FUS was able to increase CBF and alter cortical hemodynamic responses, which indicates that FUS is a potential non-invasive method to study ischemic stroke rehabilitation.
Introduction
Purpose
Transcranial ultrasound stimulation
Study Objective
To develop and demonstrate a coaxial focused ultrasound and laser speckle contrast imaging system for measuring real-time cerebral blood flow changes induced by focused ultrasound stimulation.
Animal model / Human subject
mouse, C57BL/6, 8–10 weeks, male
Disease model
Ischemic stroke
Targeted brain region(s)
Cortex
Outcomes and Safety
Summary of Outcomes
A coaxial FUS-LSCI system demonstrated that tFUS (2.9 MHz) elicits a transient increase in cerebral blood flow (115%) and vasodilation in the mouse cortex.
Duration of biological effect
60 s
Safety-related matter
The procedure was safe with a negligible temperature rise (0.047°C) and no observed vessel displacement or adverse vascular effects.
Brain Region
Ultrasound Parameters
Ultrasound instrument
coaxial FUS and laser speckle imaging system
FUS Frequency
2.9 MHz
FUS Intensity
0.51 MPa
FUS Pressure
not reported
FUS Mode
pulsed
Pulse duration
0.5 ms
Duration of a single FUS session
60 s
Treatment frequency
single session
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