Neutrophil Recruitment and Leukocyte Response Following Focused Ultrasound and Microbubble Mediated Blood-Brain Barrier Treatments.
Authors: Poon C, Pellow C, Hynynen K
(Appeared originally in Theranostics 2021; 11:1655-1671) Reprinted under Creative Commons Attribution License.
Introduction
Purpose
Drug delivery with BBB opening
Study Objective
To characterized acute leukocyte responses triggered by FUS and microbubble BBB opening and assess whether recruited leukocytes contribute to A beta plaque clearance after repeated treatments
Animal model / Human subject
EGFP Wistar rats (Wistar-TgN(CAG-GFP)184ys), male and female, 310-630 g; WT Wistar rats, 400-450 g; TgCRND* mice, 7 months old
Disease model
Healthy; Alzheimer's disease
MRI or image guidance method
Two-photon fluorescence microscopy (2PFM) image guidance
Targeted brain region(s)
Motor Cortexl Bilateral Hippocampus
Outcomes and Safety
Summary of Outcomes
FUS and microbubble BBB opening induced immediate intravascular leukocyte activity and increased neutrophils in the sonicated hemisphere; biweekly regular treatments did not significantly increase neutrophil recruitment or neutrophil A beta phagocytosis versus controls in TgCRND8 mice
Duration of biological effect
4 hours
Safety-related matter
No safety issues or adverse effects are mentioned in the provided excerpt. The supplied text contains only citation and licensing information.
Brain Region
Ultrasound Parameters
Ultrasound instrument
PZT-4 cylindrical transducer
FUS Frequency
1 MHz
FUS Pressure
0.28-0.55 Mpa
FUS Mode
pulsed
Pulse duration
10 ms
Duration of a single FUS session
120 seconds (2 minutes)
Focal Characteristics
Focal depth: None; Focal length: None; Aperture size: None
Treatment frequency
single session
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